TEMPERATURE DEPENDENCE OF ANTIBODY ADSORPTION IN PROTEIN A AFFINITY CHROMATOGRAPHY

25.08.2018

• Thermosensitive protein A ligand is characterized by flat isotherm at high temperature.
• All protein A ligands are temperature sensitive but not sufficient for temperature elution.
• Antibody bound to thermosensitive ligand can be eluted by high salt.
• Antibody undergoes reversible structural changes when it is bound to protein A medium.