WP3: Capture step
Within workpackage ''Capture step'' we will introduce the utilisation of continuous multi-column chromatography (CMC) over the standard discontinuous (''batch'') process on protein A chromatography (where the high costs of affinity resin contribute a significant fraction of the entire DSP expenditure). Alternative batch processes will also be evaluated, e.g. increased residence time (RT) and recycling of effluent back to load tank. As alternative to chromatographic separation as capture step precipitation will be evaluated also in combination with CMC on ionic exchange resin. WP3 is led by LEK Pharmaceuticals.