FACTORIZATION OF PREPARATIVE PROTEIN CHROMATOGRAMS WITH HARD-CONSTRAINT MULTIVARIATE CURVE RESOLUTION AND SECOND-DERIVATIVE PRETREATMENT

15.01.2019

Authors Matthias Rüdt, Sebastian Andris, Robin Schiemer and Jürgen Hubbuch published scientific publication entitled Factorization of preparative protein chromatograms with hard-constraint multivariate curve resolution and second-derivative pretreatment in Journal of Chromatography A.

Highlights:

  • Calibration-free factorization of multivariate UV–vis protein chromatograms.
  • Successful factorization of binary and tertiary protein chromatograms.
  • Close match of factorized chromatograms to off-line analytics.
  • The factorized spectra allowed identification of proteins from a spectral library.

Link: https://doi.org/10.1016/j.chroma.2018.11.065