FACTORIZATION OF PREPARATIVE PROTEIN CHROMATOGRAMS WITH HARD-CONSTRAINT MULTIVARIATE CURVE RESOLUTION AND SECOND-DERIVATIVE PRETREATMENT
15.01.2019
Authors Matthias Rüdt, Sebastian Andris, Robin Schiemer and Jürgen Hubbuch published scientific publication entitled Factorization of preparative protein chromatograms with hard-constraint multivariate curve resolution and second-derivative pretreatment in Journal of Chromatography A.
Highlights:
- Calibration-free factorization of multivariate UV–vis protein chromatograms.
- Successful factorization of binary and tertiary protein chromatograms.
- Close match of factorized chromatograms to off-line analytics.
- The factorized spectra allowed identification of proteins from a spectral library.
Link: https://doi.org/10.1016/j.chroma.2018.11.065