PROTEIN A AFFINITY CHROMATOGRAPHY OF CHINESE HAMSTER OVARY (CHO) CELL CULTURE BROTHS CONTAINING BIOPHARMACEUTICAL MONOCLONAL ANTIBODY (MAB): EXPERIMENTS AND MECHANISTIC TRANSPORT, BINDING AND EQUILIBRIUM MODELING

15.04.2018

Authors Matic Grom, Mirijam Kozorog, Simon Caserman, Andrej Pohar and Blaž Likozar published scientific publication entitled Protein A affinity chromatography of Chinese hamster ovary (CHO) cell culture broths containing biopharmaceutical monoclonal antibody (mAb): Experiments and mechanistic transport, binding and equilibrium modeling in Journal of Chromatography B.

Highlights:

• Protein A affinity chromatography of CHO cell culture harvest was studied.
• Properties of 5 novel resins were determined by regression on breakthrough curves.
• A mathematical model with was used to study parametric sensitivity.
• Pore diffusion is the rate limiting step in mass transfer.
• Loading residence time impacts host cell related impurities.

Link: https://doi.org/10.1016/j.jchromb.2018.02.032