SELECTIVE PROTEIN QUANTIFICATION FOR PREPARATIVE CHROMATOGRAPHY USING VARIABLE PATHLENGTH UV/VIS SPECTROSCOPY AND PARTIAL LEAST SQUARES REGRESSION

02.02.2018

Authors Nina Brestrich, Matthias Rüdt, Daniel Büchler and Jürgen Hubbuch published scientific publication entitled Selective protein quantification for preparative chromatography using variable pathlength UV/Vis spectroscopy and partial least squares regression in Chemical Engineering Science journal.

Highlights:

• PAT tool for monitoring of preparative chromatographic steps.
• In-line UV/Vis spectral measurement at protein concentrations up to 75 g/l.
• Selective protein quantification by partial least squares regression (PLS).
• Control of purification steps by calculating pool purities with PLS models.

Link: https://doi.org/10.1016/j.ces.2017.10.030